Phospholipid-dependent Ca2+ binding by the 36-kDa tyrosine kinase substrate (calpactin) and its 33-kDa core.
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چکیده
منابع مشابه
Phenothiazine binding by a homolog of calpactin, the pp60src tyrosine kinase substrate.
Microvilli isolated from 13762 mammary ascites tumor cells contain a major calcium-sensitive protein (AMV-p35) that can be isolated with microvillar microfilament cores prepared by Triton X-100 extraction in the presence but not absence of calcium. AMV-p35 can be readily purified from ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid extracts of the microfilament cores by ch...
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Gerhard Herrmann, Andreas Thiel, and Peter Böger Lehrstuhl für Physiologie und Biochemie der Pflanzen, Universität Konstanz, D-7750 Konstanz, Bundesrepublik Deutschland Z. Naturforsch. 40c, 814—818 (1985); received August 9, 1985 Photosystem-II Particles, Immunoblotting, Antibody Agglutination, Cross Reaction, Urea-Gradient Gel Electrophoresis A rabbit antiserum was prepared against the purifie...
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We have purified three 35-kDa calcium- and phospholipid-binding proteins from rat liver. These three calcimedins bind to phosphatidylserine in a calcium-dependent manner and have been termed 35 alpha, 35 beta, and 35 gamma based on their relative charge as determined by isoelectric focusing. Purification of the three 35-kDa calcimedins is achieved by phenyl-Sepharose, ion exchange, and gel filt...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1986
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)38382-5